Gene. 1986;41(2-3):311-4.

Isolation and characterization of human blood-coagulation factor X cDNA.

Kaul RK, Hildebrand B, Roberts S, Jagadeeswaran P.

Abstract

Using synthetic oligodeoxynucleotides as probes, we have isolated factor X cDNA from human liver cDNA library. We sequenced the 1430-bp cDNA which spans the coding region of the mature factor X and contains the polyadenylation signal and poly(A) tail. The amino acid (aa) sequence is in agreement with the published aa sequence. The nucleotide (nt) sequence of cDNA confirmed that factor X is synthesized and secreted as a single-chain precursor, and then converted into dimeric form by proteolytic cleavage of an internal tripeptide. From the nt sequence, it was also predicted that like other secretory proteins, human factor X is synthesized with a leader sequence (prepro-protein). The 5'-coding region of factor X cDNA is 60 and 40% homologous to the corresponding regions of factor IX and prothrombin genes, respectively. This supports the hypothesis of gene evolution by gene duplication followed by divergence.

PMID:: 3011603; [PubMed - indexed for MEDLINE]

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GENBANK/M22613

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HL31458/HL/NHLBI NIH HHS/United States

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Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence. [Nucleic Acids Res. 1984]
Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence.
Fung MR, Campbell RM, MacGillivray RT. Nucleic Acids Res. 1984 Jun 11; 12(11):4481-92.
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Cited by 2 PubMed Central articles

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