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Molecular cloning and sequence analysis of human Na,K-ATPase beta-subunit.
We have isolated a cDNA clone for the beta-subunit of HeLa cell Na,K-ATPase, containing a 2208-base-pair cDNA insert covering the whole coding region of the beta-subunit. Nucleotide sequence analysis revealed that the amino acid sequence of human Na,K-ATPase exhibited 61% homology with that of Torpedo counterpart (Noguchi et al. (1986) FEBS Lett. in press). A remarkable conservation in the nucleotide sequence of the 3' non-coding region was detected between the human and Torpedo cDNAs. RNA blot hybridization analysis revealed the presence of two mRNA species in HeLa cells. S1 nuclease mapping indicated that they were derived from utilization of two distinct polyadenylation signals in vivo. Total genomic Southern hybridization indicated the existence of only a few, possibly one set of gene encoding the Na,K-ATPase beta-subunit in the human genome.
PMID: 3008098 [PubMed - indexed for MEDLINE]
PMCID: PMC339706
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Cited by 14 PubMed Central articles
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Multiple genes for essential-hypertension susceptibility on chromosome 1q.
Chang YP, Liu X, Kim JD, Ikeda MA, Layton MR, Weder AB, Cooper RS, Kardia SL, Rao DC, Hunt SC, et al.
Am J Hum Genet. 2007 Feb; 80(2):253-64. Epub 2006 Dec 20.
[Am J Hum Genet. 2007]
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Molecular and functional studies of electrogenic Na(+) transport in the distal colon and rectum of young and elderly subjects.
Greig ER, Mathialahan T, Boot-Handford RP, Sandle GI.
Gut. 2003 Nov; 52(11):1607-15.
[Gut. 2003]
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Partitioning of tissue expression accompanies multiple duplications of the Na+/K+ ATPase alpha subunit gene.
Serluca FC, Sidow A, Mably JD, Fishman MC.
Genome Res. 2001 Oct; 11(10):1625-31.
[Genome Res. 2001]
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Patient Drug Information
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Potassium (Glu-K® , K+ 10® , K+ 8® , ...)
Potassium is essential for the proper functioning of the heart, kidneys, muscles, nerves, and digestive system. Usually the food you eat supplies all of the potassium you need. However, certain diseases (e.g., kidney dis...