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Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).
Kawasaki ES,
Ladner MB,
Wang AM,
Van Arsdell J,
Warren MK,
Coyne MY,
Schweickart VL,
Lee MT,
Wilson KJ,
Boosman A, et al.
Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids. This cDNA, which was cloned in the Okayama-Berg expression vector, specifies the synthesis of biologically active CSF-1 in COS cells, as determined by a specific radioreceptor assay, macrophage bone marrow colony formation, and antibody neutralization. Most of the cDNA isolates contain part of an intron sequence that changes the reading frame, resulting in an abrupt termination of translation; these cDNA's were inactive in COS cells. The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases.
PMID: 2996129 [PubMed - indexed for MEDLINE]
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Cited by 70 PubMed Central articles
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ReviewHematopoietic cytokines.
Metcalf D.
Blood. 2008 Jan 15; 111(2):485-91.
[Blood. 2008]
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Roles of adeno-associated virus Rep protein and human chromosome 19 in site-specific recombination.
Young SM Jr, McCarty DM, Degtyareva N, Samulski RJ.
J Virol. 2000 May; 74(9):3953-66.
[J Virol. 2000]
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Heterozygous osteopetrotic (op) mutation reduces atherosclerosis in LDL receptor- deficient mice.
Rajavashisth T, Qiao JH, Tripathi S, Tripathi J, Mishra N, Hua M, Wang XP, Loussararian A, Clinton S, Libby P, et al.
J Clin Invest. 1998 Jun 15; 101(12):2702-10.
[J Clin Invest. 1998]
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