Purification, crystallization and preliminary X-ray crystallographic studies of swine MHC class I complexed with an FMDV CTL epitope Hu64

Lei Feng; Ming-Wei Sun; Ping Jiang; Zi-Bin Li; Hua Gao; Xiao-Xin Zhai; Yong Han; Zong-Hui Zhang; Feng-Shan Gao

doi:10.1016/j.rvsc.2018.06.018

Purification, crystallization and preliminary X-ray crystallographic studies of swine MHC class I complexed with an FMDV CTL epitope Hu64

Res Vet Sci. 2018 Aug:119:205-208. doi: 10.1016/j.rvsc.2018.06.018. Epub 2018 Jun 20.

Authors

Lei Feng¹, Ming-Wei Sun², Ping Jiang³, Zi-Bin Li⁴, Hua Gao³, Xiao-Xin Zhai³, Yong Han³, Zong-Hui Zhang³, Feng-Shan Gao⁵

Affiliations

¹ Department of Bioengineering, College of Life Science and Technology, Dalian University, Dalian, Liaoning 116622, PR China; CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, PR China.
² CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, PR China.
³ Department of Bioengineering, College of Life Science and Technology, Dalian University, Dalian, Liaoning 116622, PR China.
⁴ Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, PR China.
⁵ Department of Bioengineering, College of Life Science and Technology, Dalian University, Dalian, Liaoning 116622, PR China. Electronic address: gfsh0626@126.com.

PMID: 29958155
DOI: 10.1016/j.rvsc.2018.06.018

Abstract

Up to now, no crystal structure of swine leukocyte antigen 2 (SLA-2) molecules was reported. In order to elucidate the structure of SLA-2 and to study the cytotoxic T lymphocyte (CTL) epitopes derived from foot-and-mouth disease virus (FMDV), a complex of swine major histocompatibility complex (MHC) class I molecule (SLA-2 haplotype, Hebao allele) with swine β₂-microglobulin and the CTL epitope FMDV-Hu64 (ALLRTATYY) derived from O serotype of FMDV VP1 protein (residues 64-72) was refolded and crystallized. The crystal, which belonged to space group P2₁2₁2₁, diffracted to 2.5 Å resolution and had unit cell parameters a = 48.37, b = 97.75, c = 166.163 Å. These results will help to determine the first structure of a SLA-2 molecule in the context of an FMDV CTL epitope.

Keywords: Crystallization; Cytotoxic T lymphocyte; Epitope; FMDV; SLA-2.

MeSH terms

Animals
Crystallization
Epitopes, T-Lymphocyte / chemistry*
Foot-and-Mouth Disease Virus*
Histocompatibility Antigens Class I / chemistry*
Swine*
X-Rays

Substances

Epitopes, T-Lymphocyte
Histocompatibility Antigens Class I