Multi-isotope provenancing of archaeological skeletons including cremations in a reference area of the European Alps

Rationale: Due to the spatial heterogeneity of stable isotope ratios of single elements measured in attempts to georeference bioarchaeological finds, multi-isotope fingerprints are frequently employed under the assumption that similar isotopic signatures are indicative of similar shared environments by the individuals studied. The extraction of the spatial information from multi-isotope datasets, however, is challenging.

Methods: Gaussian mixture clustering of six- to seven-dimensional isotopic fingerprints measured in archaeological animal and human bones was performed. Uncremated animal bones served for an isotopic mapping of a specific reference area of eminent archaeological importance, namely the Inn-Eisack-Adige passage across the European Alps. The fingerprints consist of ⁸⁷ Sr/⁸⁶ Sr, ²⁰⁸ Pb/²⁰⁴ Pb, ²⁰⁷ Pb/²⁰⁴ Pb, ²⁰⁶ Pb/²⁰⁴ Pb, ²⁰⁸ Pb/²⁰⁷ Pb, and ²⁰⁶ Pb/²⁰⁷ Pb ratios, and δ¹⁸ O_phosphate values in uncremated bone apatite, while the thermally unstable δ¹⁸ O values of human cremations from this region were discarded.

Results: The bone finds were successfully decontaminated. Animal and human isotope clusters not only reflect individual similarities in the multi-isotopic fingerprints, but also permit a spatial allocation of the finds. This holds also for cremated finds where the δ¹⁸ O_phosphate value is no longer informative. To our knowledge, for the first time Pb stable isotopes have been systematically studied in cremated skeletal remains and proved significant in a region that was sought after for its ore deposits in prehistory.

Conclusions: Gaussian mixture clustering is a promising method for the interpretation of multi-isotopic fingerprints aiming at detecting and quantifying migration and trade.