Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation

Appl Environ Microbiol. 1985 Jul;50(1):169-71. doi: 10.1128/aem.50.1.169-171.1985.

Abstract

A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cloning, Molecular*
  • DNA Transposable Elements
  • Electrophoresis, Agar Gel
  • Genes, Bacterial
  • Genetic Complementation Test
  • Mutation
  • Plasmids*
  • Pseudomonas / genetics*

Substances

  • DNA Transposable Elements