Isolation of a mouse DNA fragment with homology to a Drosophila ribosomal protein gene

Gene. 1985;34(2-3):155-61. doi: 10.1016/0378-1119(85)90123-4.

Abstract

A mouse genomic library in lambda Charon 4A was screened for putative ribosomal protein genes using a fragment of the gene encoding Drosophila ribosomal protein 49 as a hybridization probe under nonstringent hybridization conditions. A recombinant phage was selected and its restriction enzyme map determined. The major species of mouse poly(A)+ mRNA homologous to the putative gene is about 740 nucleotides long.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biological Evolution
  • Chromosome Mapping
  • DNA Restriction Enzymes
  • Drosophila melanogaster / genetics*
  • Genes
  • Mice
  • Nucleic Acid Hybridization
  • Poly A / genetics
  • RNA, Messenger / genetics
  • Ribosomal Proteins / genetics*

Substances

  • RNA, Messenger
  • Ribosomal Proteins
  • Poly A
  • DNA Restriction Enzymes