Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation

Svitlana V Malysheva; Stephanie Wunderlich; Alexandra Haase; Gudrun Göhring; Ulrich Martin; Sylvia Merkert

doi:10.1016/j.scr.2018.05.015

Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation

Stem Cell Res. 2018 Jul:30:117-121. doi: 10.1016/j.scr.2018.05.015. Epub 2018 May 19.

Authors

Svitlana V Malysheva¹, Stephanie Wunderlich¹, Alexandra Haase¹, Gudrun Göhring², Ulrich Martin¹, Sylvia Merkert³

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany.
² REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.
³ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany. Electronic address: merkert.sylvia@mh-hannover.de.

PMID: 29852468
DOI: 10.1016/j.scr.2018.05.015

Abstract

Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2^pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2^Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

CDX2 Transcription Factor / metabolism*
Cell Differentiation
Female
Humans
Induced Pluripotent Stem Cells / metabolism*
Infant, Newborn
Trophoblasts / cytology
Trophoblasts / metabolism

Substances

CDX2 Transcription Factor