Visfatin increases miR-21 to promote migration in HCC

Cell Mol Biol (Noisy-le-grand). 2018 May 15;64(6):48-52.

Abstract

Hepatocellular carcinoma (HCC) is a common human malignancy. In this study, we aimed to investigate the serum levels of visfatin and miR-21 in HCC patients, to analysis the relationship between the pathological features and the plasma level of visfatin or miR-21, and to explore the roles of visfatin and miR-21 in migration of HCC cells. Our results showed that the serum levels of visfatin and miR-21 were significant higher in HCC patients than healthy subjects. The diagnostic sensitivity of serum visfatin was 82.5% and the specificity was 65.0%. The serum visfatin was significantly associated with the histology and metastasis. Visfatin induced miR-21 expression and cell migration in HepG2 cells. Transfection of miR-21 inhibitor suppressed the visfatin-induced migration in HCC cells. These results suggested that visfatin induced HCC cell migration via upregulation of miR-21, which provides a novel basis for the diagnosis of HCC.

Keywords: HCC; HepG2; Metastasis.; Visfatin; miR-21.

MeSH terms

  • Abdominal Fat / metabolism
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood
  • Carcinoma, Hepatocellular / blood
  • Carcinoma, Hepatocellular / diagnosis
  • Carcinoma, Hepatocellular / epidemiology
  • Carcinoma, Hepatocellular / pathology*
  • Cytokines / blood
  • Cytokines / metabolism
  • Cytokines / physiology*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms / blood
  • Liver Neoplasms / diagnosis
  • Liver Neoplasms / epidemiology
  • Liver Neoplasms / pathology*
  • Male
  • MicroRNAs / biosynthesis
  • MicroRNAs / blood
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Middle Aged
  • Neoplasm Metastasis
  • Neoplasm Proteins / blood
  • Neoplasm Proteins / metabolism
  • Neoplasm Proteins / physiology*
  • Nicotinamide Phosphoribosyltransferase / blood
  • Nicotinamide Phosphoribosyltransferase / metabolism
  • Nicotinamide Phosphoribosyltransferase / physiology*
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / blood
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / physiology*
  • Recombinant Proteins / metabolism
  • Sensitivity and Specificity
  • Up-Regulation

Substances

  • Biomarkers, Tumor
  • Cytokines
  • MIRN21 microRNA, human
  • MicroRNAs
  • Neoplasm Proteins
  • RNA, Neoplasm
  • Recombinant Proteins
  • Nicotinamide Phosphoribosyltransferase
  • nicotinamide phosphoribosyltransferase, human