Abstract
Hepatocellular carcinoma (HCC) is a common human malignancy. In this study, we aimed to investigate the serum levels of visfatin and miR-21 in HCC patients, to analysis the relationship between the pathological features and the plasma level of visfatin or miR-21, and to explore the roles of visfatin and miR-21 in migration of HCC cells. Our results showed that the serum levels of visfatin and miR-21 were significant higher in HCC patients than healthy subjects. The diagnostic sensitivity of serum visfatin was 82.5% and the specificity was 65.0%. The serum visfatin was significantly associated with the histology and metastasis. Visfatin induced miR-21 expression and cell migration in HepG2 cells. Transfection of miR-21 inhibitor suppressed the visfatin-induced migration in HCC cells. These results suggested that visfatin induced HCC cell migration via upregulation of miR-21, which provides a novel basis for the diagnosis of HCC.
Keywords:
HCC; HepG2; Metastasis.; Visfatin; miR-21.
MeSH terms
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Abdominal Fat / metabolism
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Adult
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Aged
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Aged, 80 and over
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Biomarkers, Tumor / blood
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Carcinoma, Hepatocellular / blood
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Carcinoma, Hepatocellular / diagnosis
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Carcinoma, Hepatocellular / epidemiology
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Carcinoma, Hepatocellular / pathology*
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Cytokines / blood
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Cytokines / metabolism
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Cytokines / physiology*
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Female
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Gene Expression Regulation, Neoplastic
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Hep G2 Cells
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Humans
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Liver Neoplasms / blood
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Liver Neoplasms / diagnosis
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Liver Neoplasms / epidemiology
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Liver Neoplasms / pathology*
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Male
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MicroRNAs / biosynthesis
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MicroRNAs / blood
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MicroRNAs / genetics
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MicroRNAs / physiology*
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Middle Aged
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Neoplasm Metastasis
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Neoplasm Proteins / blood
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Neoplasm Proteins / metabolism
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Neoplasm Proteins / physiology*
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Nicotinamide Phosphoribosyltransferase / blood
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Nicotinamide Phosphoribosyltransferase / metabolism
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Nicotinamide Phosphoribosyltransferase / physiology*
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / blood
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RNA, Neoplasm / genetics
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RNA, Neoplasm / physiology*
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Recombinant Proteins / metabolism
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Sensitivity and Specificity
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Up-Regulation
Substances
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Biomarkers, Tumor
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Cytokines
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MIRN21 microRNA, human
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MicroRNAs
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Neoplasm Proteins
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RNA, Neoplasm
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Recombinant Proteins
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Nicotinamide Phosphoribosyltransferase
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nicotinamide phosphoribosyltransferase, human