Monocytic myeloid-derived suppressor cells generated from rhesus macaque bone marrow enrich for regulatory T cells

Cell Immunol. 2018 Jul:329:50-55. doi: 10.1016/j.cellimm.2018.04.013. Epub 2018 Apr 27.

Abstract

Putative monocytic myeloid-derived suppressor cells (mMDSC; lineage-HLA-DR-/lo) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment of the capacity of these subsets to suppress anti-CD3/CD28-stimulated CD4 and CD8 T cell proliferation revealed that the most potent population was CD14hiCD33-/loCD34loCD11bhi. These BM-derived mMDSC markedly increased the incidence of CD4+CD25+CD127-Foxp3+ regulatory T cells in responder T cell populations. They offer potential value in testing the therapeutic efficacy of immunoregulatory mMDSC for the promotion of tolerance in nonhuman primate transplant models.

Keywords: Bone marrow; Immune regulation; Myeloid-derived suppressor cells; Nonhuman primate; T cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biomarkers
  • Bone Marrow / pathology
  • Bone Marrow / physiology
  • Bone Marrow Cells / cytology*
  • Flow Cytometry
  • Macaca mulatta
  • Male
  • Monocytes / physiology
  • Myeloid Cells / physiology
  • Myeloid-Derived Suppressor Cells / metabolism
  • Myeloid-Derived Suppressor Cells / physiology*
  • Primary Cell Culture / methods
  • T-Lymphocyte Subsets / physiology
  • T-Lymphocytes, Regulatory / physiology*

Substances

  • Biomarkers