Chromatography of oxysterols

Irundika H K Dias; Steven R Wilson; Hanne Roberg-Larsen

doi:10.1016/j.biochi.2018.05.004

Chromatography of oxysterols

Biochimie. 2018 Oct:153:3-12. doi: 10.1016/j.biochi.2018.05.004. Epub 2018 May 9.

Authors

Irundika H K Dias¹, Steven R Wilson², Hanne Roberg-Larsen³

Affiliations

¹ Aston Medical Research Institute, Aston Medical School, Aston University, Birmingham, B4 7ET, UK.
² Department of Chemistry, University of Oslo, PO Box 1033, Blindern, NO-0315, Oslo, Norway.
³ Department of Chemistry, University of Oslo, PO Box 1033, Blindern, NO-0315, Oslo, Norway. Electronic address: hanne.roberg-larsen@kjemi.uio.no.

PMID: 29751082
DOI: 10.1016/j.biochi.2018.05.004

Abstract

Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

Keywords: Chromatography; Lipids; Oxysterols; Separation; Sterols.

Publication types

Review

MeSH terms

Chromatography, Gas / methods*
Chromatography, Liquid / methods*
Oxysterols / isolation & purification*

Substances

Oxysterols