Chlorate: a reversible inhibitor of proteoglycan sulfation

Biochem Biophys Res Commun. 1988 Jul 15;154(1):365-71. doi: 10.1016/0006-291x(88)90694-8.

Abstract

Bovine aorta endothelial cells were cultured in medium containing [3H]glucosamine, [35S]sulfate, and various concentrations of chlorate. Cell growth was not affected by 10 mM chlorate, while 30 mM chlorate had a slight inhibitory effect. Chlorate concentrations greater than 10 mM resulted in significant undersulfation of chondroitin. With 30 mM chlorate, sulfation of chondroitin was reduced to 10% and heparan to 35% of controls, but [3H]glucosamine incorporation on a per cell basis did not appear to be inhibited. Removal of chlorate from the culture medium of cells resulted in the rapid resumption of sulfation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cells, Cultured
  • Chlorates / pharmacology*
  • Chondroitin Sulfate Proteoglycans / biosynthesis*
  • Endothelium, Vascular / metabolism*
  • Glucosamine / metabolism
  • Glycosaminoglycans / biosynthesis*
  • Heparitin Sulfate / biosynthesis*
  • Kinetics
  • Proteoglycans / biosynthesis*
  • Sulfates / metabolism*
  • Sulfur Radioisotopes
  • Tritium

Substances

  • Chlorates
  • Chondroitin Sulfate Proteoglycans
  • Glycosaminoglycans
  • Proteoglycans
  • Sulfates
  • Sulfur Radioisotopes
  • Tritium
  • Heparitin Sulfate
  • Glucosamine