Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro

Shi-Fang Li; Jun-Jun Shao; Fu-Rong Zhao; Mei-Jiao Gong; Yin-Li Xie; Hui-Yun Chang; Yong-Guang Zhang

doi:10.1016/j.intimp.2018.03.022

Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro

Int Immunopharmacol. 2018 Jun:59:47-52. doi: 10.1016/j.intimp.2018.03.022. Epub 2018 Apr 3.

Authors

Shi-Fang Li¹, Jun-Jun Shao¹, Fu-Rong Zhao¹, Mei-Jiao Gong², Yin-Li Xie¹, Hui-Yun Chang³, Yong-Guang Zhang⁴

Affiliations

¹ State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, PR China.
² State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China; College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
³ State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, PR China. Electronic address: changhuiyun@caas.cn.
⁴ State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, PR China. Electronic address: zhangyongguang@caas.cn.

PMID: 29621736
DOI: 10.1016/j.intimp.2018.03.022

Abstract

Foot-and-mouth disease (FMD) is one of the most devastating diseases affecting livestock. Since vaccines fail to provide protection until seven days post-vaccination, the application of anti-viral molecules is imperative for suppressing the spread of FMDV prior to development of an adaptive immune response. Interferons (IFNs) are effective for the host to fight FMDV infections; however, a novel type I IFNs, interferon delta (IFN-δ), has not been investigated for their antiviral effects against this virus. Thus, this study investigated FMDV infection, upon pre- and post-treatment with PoIFN-δ8. Real-time quantitative PCR was used to quantify the expression levels of IFN-stimulated genes (ISGs), including ISG15, OAS1, PKR, and Mx1. Results showed the PoIFN-δ8 lacking its signal sequence was efficiently expressed in Escherichia coli, and the purified recombinant PoIFN-δ8 exerted a significantly protective effect against two different serotypes of FMDV in IBRS-2 cells. In addition, PoIFN-δ8 induced the expression of IFN-stimulated genes. These findings highlight the significance of PoIFN-δ might serve as an antiviral agent for the prevention of FMDV in pigs and will stimulate the study of exploiting the potential biological functions of IFN-δ in the future.

Keywords: Antiviral activity; FMDV; Porcine interferon-delta; Type I interferon.

MeSH terms

Animals
Antiviral Agents / pharmacology*
Cell Line
Foot-and-Mouth Disease Virus / drug effects*
Interferon Type I / pharmacology*
Swine

Substances

Antiviral Agents
Interferon Type I
interferon, delta