Intrathecal Administration of CXCL1 Enhances Potassium Currents in Microglial Cells

Alexandru-Florian Deftu; Violeta Ristoiu; Marc Rene Suter

doi:10.1159/000486865

Intrathecal Administration of CXCL1 Enhances Potassium Currents in Microglial Cells

Pharmacology. 2018;101(5-6):262-268. doi: 10.1159/000486865. Epub 2018 Feb 15.

Authors

Alexandru-Florian Deftu¹, Violeta Ristoiu¹, Marc Rene Suter²

Affiliations

¹ Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Bucharest, Romania.
² Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland.

PMID: 29448255
DOI: 10.1159/000486865

Abstract

The functioning of microglial cells inside the central nervous system depends on their ion channels expression. Microglia are capable of synthesizing different cytokines and chemokines, including CXCL1, and responding to their action via specific receptors. In this study, we explore the effect of intrathecal injection of CXCL1 on potassium currents, expressed in CX3CR1-Green Fluorescent Protein labeled microglia in transgenic mice. The results showed that CXCL1 hyperpolarized the cells by enhancing inward rectifying potassium currents and increasing the membrane area, suggesting an activating effect on microglia.

Keywords: CXCL1; Microglia; Potassium currents.

MeSH terms

Animals
CX3C Chemokine Receptor 1 / genetics
Chemokine CXCL1 / administration & dosage*
Green Fluorescent Proteins / genetics
Injections, Spinal
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microglia / metabolism*
Potassium Channels, Inwardly Rectifying / metabolism*

Substances

CX3C Chemokine Receptor 1
Chemokine CXCL1
Cx3cr1 protein, mouse
Potassium Channels, Inwardly Rectifying
enhanced green fluorescent protein
Green Fluorescent Proteins