The first complete genome sequence of narcissus latent virus from Narcissus

Arch Virol. 2018 May;163(5):1383-1386. doi: 10.1007/s00705-018-3741-x. Epub 2018 Feb 1.

Abstract

The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge, this is the first report of the complete sequence of a NLV isolate.

MeSH terms

  • Capsid Proteins / genetics
  • Genome, Viral*
  • Narcissus / virology*
  • Netherlands
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology
  • Potyviridae / genetics*
  • RNA, Viral / genetics
  • Sequence Analysis, DNA
  • Whole Genome Sequencing

Substances

  • Capsid Proteins
  • RNA, Viral