Selected steps of swine ICSI method to overcome seasonal effect and achieve acceptable early embryonic development, a preliminary study

I A Tsakmakidis; M Tsantarliotou; I A Michos; A G Basioura; G Tsousis; C M Boscos

doi:10.1515/pjvs-2017-0075

Selected steps of swine ICSI method to overcome seasonal effect and achieve acceptable early embryonic development, a preliminary study

Pol J Vet Sci. 2017 Sep 26;20(3):607-609. doi: 10.1515/pjvs-2017-0075.

Authors

I A Tsakmakidis¹, M Tsantarliotou¹, I A Michos¹, A G Basioura¹, G Tsousis¹, C M Boscos¹

Affiliation

¹ .

PMID: 29166269
DOI: 10.1515/pjvs-2017-0075

Abstract

Porcine intra cytoplasmic sperm injection's (ICSI) efficacy by selected protocol steps was investigated. Three trials per year's period (hot, medium, cold) were carried out. Only large size follicles (6-8mm) were aspirated, brilliant cresyl blue (BCB) test was performed and only the BCB+ oocytes were in vitro maturated (40h) and involved to ICSI process. The presumptive embryos were in vitro cultured (15h). Raw boar semen and SpermCatch® as slowing medium were used. No differences were observed between periods regarding early embryonic development and maturation competence. ICSI achieves acceptable porcine early embryonic development rates under the investigated conditions.

Keywords: BCB stain; ICSI; boar sperm; pig embryos; pig oocytes.

MeSH terms

Animals
Embryo Culture Techniques / veterinary*
Embryonic Development
Female
In Vitro Oocyte Maturation Techniques
Male
Oocytes
Seasons*
Sperm Injections, Intracytoplasmic / veterinary*
Swine*