Beta-endorphin modification by transglutaminase in vitro: identification by FAB/MS of glutamine-11 and lysine-29 as acyl donor and acceptor sites

Biochem Biophys Res Commun. 1988 Jul 29;154(2):735-40. doi: 10.1016/0006-291x(88)90201-x.

Abstract

FAB-Mapping strategy was successfully exploited to characterize the reaction products of the transglutaminase-mediated modifications of human beta-endorphin in vitro. The GLN-11 residue of the neuropeptide was shown to be an effective acyl donor site for the enzyme, being able to bind spermine in the presence of Ca2+. Moreover, only one out of five lysyl residues (LYS-29) was demonstrated to act as acyl acceptor crosslinking with GLN-11.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Amino Acid Sequence
  • Glutamine / analysis*
  • Humans
  • Lysine / analysis*
  • Mass Spectrometry
  • Molecular Sequence Data
  • Transglutaminases / metabolism*
  • beta-Endorphin / pharmacology*

Substances

  • Glutamine
  • beta-Endorphin
  • Transglutaminases
  • Lysine