New murine homeoboxes: structure, chromosomal assignment, and differential expression in adult erythropoiesis

DNA. 1987 Oct;6(5):409-18. doi: 10.1089/dna.1987.6.409.

Abstract

The nucleotide sequence, chromosomal assignment, and preliminary transcriptional analysis of four murine homeoboxes is presented. Three of these are linked to the Hox-2 gene complex on chromosome 11, whereas the fourth, Hox-4, was assigned to mouse chromosome 12. A comparative analysis of homeobox sequences reveals that two of our sequences represent the previously described Hox-2.3 loci, whereas a third, mh19, could represent the predicted Hox-2.6 locus. Homeoboxes Hox-2.2 and Hox-2.3 are the cognates of two previously reported human homeoboxes that belong to a similar gene cluster on a closely related human chromosome (Chr 17), suggesting that homeoboxes may have been preserved as clusters during evolution. Moreover Hox-4, mh19, and the previously described Hox-1.5 homeobox form a separate subgroup of mammalian homeoboxes (90-92% amino acid and nucleotide homology). All four homeoboxes are expressed in the mouse embryo. Of special interest is the expression of mh19, a 4.2-kb transcript of which appears to be connected to the induced differentiation of Friend erythroleukemia cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Erythropoiesis*
  • Gene Expression Regulation
  • Genes, Homeobox*
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Species Specificity

Associated data

  • GENBANK/M18166
  • GENBANK/M18167
  • GENBANK/M18168
  • GENBANK/M18169