Sea urchin actin gene linkages determined by genetic segregation

Dev Biol. 1987 Jul;122(1):291-5. doi: 10.1016/0012-1606(87)90354-x.

Abstract

Genetic linkage between the actin genes of Strongylocentrotus purpuratus was investigated by observing the segregation of restriction fragment length polymorphisms (RFLPs). Specific RFLPs of actin gene pairs CyI/CyIIa and CyIIIa/CyIIIb always cosegregated, confirming the linkage groups CyI-CyIIa-CyIIb previously previously determined by molecular cloning. In contrast, RFLPs of actin genes CyI/CyIIa, CyIIIa/CyIIIb, and M all segregated at random with respect to one another. This demonstrates that the known actin gene clusters CyI-CyIIa-CyIIb, CyIIIa-CyIIIb, and the M actin gene are not closely linked.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics*
  • Animals
  • DNA / genetics
  • Female
  • Genetic Linkage*
  • Heterozygote
  • Male
  • Polymorphism, Restriction Fragment Length
  • Sea Urchins / genetics*

Substances

  • Actins
  • DNA