Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae

Ana C Ramos; Ana C Gales; Jussimara Monteiro; Suzane Silbert; Thomás Chagas-Neto; Antonia M O Machado; Cecilia G Carvalhaes

doi:10.1016/j.mimet.2017.08.016

Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae

J Microbiol Methods. 2017 Nov:142:1-3. doi: 10.1016/j.mimet.2017.08.016. Epub 2017 Aug 24.

Authors

Ana C Ramos¹, Ana C Gales², Jussimara Monteiro³, Suzane Silbert⁴, Thomás Chagas-Neto⁵, Antonia M O Machado⁵, Cecilia G Carvalhaes⁶

Affiliations

¹ Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: anarolramos@msn.com.
² Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil.
³ Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
⁴ Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States.
⁵ Laboratório Central, Hospital São Paulo, Division of Laboratory Medicine, Departament of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil.
⁶ Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil; Laboratório Central, Hospital São Paulo, Division of Laboratory Medicine, Departament of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil.

PMID: 28844719
DOI: 10.1016/j.mimet.2017.08.016

Abstract

The rapid detection of KPC-producing Enterobacteriaceae by microbiology laboratories has been required for infectious control programs. Herein we evaluated the performance of a novel immunochromatographic test for detecting KPC-2-, KPC-3-, KPC-4-, KPC-6-, KPC-7-, KPC-8-, and KPC-11-producing isolates and the influence of different growth media on the test performance.

Keywords: Chromogenic agar; Culture medium; KPC variants; KPC-K-SeT; MacConkey; Mueller-Hinton.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics*
Carbapenem-Resistant Enterobacteriaceae / enzymology
Carbapenem-Resistant Enterobacteriaceae / genetics*
Carbapenem-Resistant Enterobacteriaceae / isolation & purification
Chromatography, Affinity / methods*
Enterobacteriaceae Infections / microbiology
Humans
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Microbial Sensitivity Tests
beta-Lactamases / genetics*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase