Enhancer Analyses Using Chicken Embryo Electroporation

Methods Mol Biol. 2017:1650:191-202. doi: 10.1007/978-1-4939-7216-6_12.

Abstract

Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region.

Keywords: Core region; Electroporation; Enhancers; Transcription factor binding; tkEGFP.

MeSH terms

  • Animals
  • Chick Embryo
  • Chickens / genetics*
  • Electroporation / methods*
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins / metabolism
  • Promoter Regions, Genetic*

Substances

  • Green Fluorescent Proteins