Enhanced biobutanol production with high yield from crude glycerol by acetone uncoupled Clostridium sp. strain CT7

Fengxue Xin; Tianpeng Chen; Yujia Jiang; Jiasheng Lu; Weiliang Dong; Wenming Zhang; Jiangfeng Ma; Min Zhang; Min Jiang

doi:10.1016/j.biortech.2017.08.002

Enhanced biobutanol production with high yield from crude glycerol by acetone uncoupled Clostridium sp. strain CT7

Bioresour Technol. 2017 Nov;244(Pt 1):575-581. doi: 10.1016/j.biortech.2017.08.002. Epub 2017 Aug 5.

Authors

Fengxue Xin¹, Tianpeng Chen², Yujia Jiang², Jiasheng Lu², Weiliang Dong¹, Wenming Zhang¹, Jiangfeng Ma¹, Min Zhang¹, Min Jiang³

Affiliations

¹ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China.
² State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China.
³ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China. Electronic address: bioengine@njtech.edu.cn.

PMID: 28803108
DOI: 10.1016/j.biortech.2017.08.002

Abstract

This study reports a unique acetone uncoupled Clostridium species strain CT7, which shows efficient capability of glycerol utilization with high butanol ratio. Medium compositions, such as substrate concentration, micronutrients and pH show significant effects on butanol production from glycerol by strain CT7. To further maximize butanol production, fermentation conditions were optimized by using response surface methodology (RSM). Final butanol production of 16.6g/L with yield of 0.43g/g consumed glycerol was obtained, representing the highest butanol production and yield from glycerol in the batch fermentation mode. Furthermore, strain CT7 could directly convert crude glycerol to 11.8g/L of butanol without any pretreatment. Hence, strain CT7 shows immense potential for biofuels production using waste glycerol as cheap substrate.

Keywords: Biobutanol; By-products; Clostridium; Crude glycerol; Response surface methodology.

MeSH terms

1-Butanol
Acetone*
Butanols
Clostridium / metabolism*
Fermentation
Glycerol*

Substances

Butanols
Acetone
1-Butanol
Glycerol