Synthesis of Biocompatible Hydroxyapatite Using Chitosan Oligosaccharide as a Template

Jinyu Wang; Guanxiong Liu; Jinshuai Chen; Bo Zhao; Peizhi Zhu

doi:10.3390/ma8125440

Synthesis of Biocompatible Hydroxyapatite Using Chitosan Oligosaccharide as a Template

Materials (Basel). 2015 Nov 30;8(12):8097-8105. doi: 10.3390/ma8125440.

Authors

Jinyu Wang¹, Guanxiong Liu², Jinshuai Chen³, Bo Zhao⁴, Peizhi Zhu⁵

Affiliations

¹ School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. jinywang2@gmail.com.
² School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. liugangx130@gmail.com.
³ School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. chenjsu7@gmail.com.
⁴ Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. zhaobo@njnu.edu.cn.
⁵ School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. pzzhu@yzu.edu.cn.

Abstract

In this study, a novel biocompatible hydroxyapatite (HA) was synthesized by using chitosan oligosaccharide (COS) as a template. These HA samples were studied by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The biocompatibility of HA samples was evaluated via cell viability, cell morphology and alkaline phosphatase staining of MG-63 cell lines. The results show that HA synthesized in the presence of COS was favorable to proliferation and osteogenic differentiation of MG-63 cells. These hydroxyapatites are potentially attractive biomaterials for bone tissue engineering applications.

Keywords: MG-63 cell; chitosan oligosaccharide; cytotoxicity; hydroxyapatite.