Abstract
A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Clostridium / genetics*
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Clostridium perfringens / genetics*
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DNA Restriction Enzymes
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DNA, Bacterial / isolation & purification*
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Electrophoresis, Agar Gel
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Hydrogen-Ion Concentration
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Plasmids*
Substances
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DNA, Bacterial
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DNA Restriction Enzymes