Transformation of Schizosaccharomyces pombe in a 96-Well Format

Assen Roguev; Jiewei Xu; Nevan J Krogan

doi:10.1101/pdb.prot091942

Transformation of Schizosaccharomyces pombe in a 96-Well Format

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot091942.

Authors

Assen Roguev¹, Jiewei Xu², Nevan J Krogan²

Affiliations

¹ Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518 roguev@gmail.com.
² Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518.

PMID: 28733397
DOI: 10.1101/pdb.prot091942

Abstract

This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.

MeSH terms

Genetics, Microbial / methods*
High-Throughput Screening Assays / methods
Molecular Biology / methods*
Schizosaccharomyces / genetics*
Transformation, Genetic*