RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA

Rosanna Tofalo; Aldo Corsetti

doi:10.1007/978-1-4939-7187-9_18

RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA

Methods Mol Biol. 2017:1644:195-201. doi: 10.1007/978-1-4939-7187-9_18.

Authors

Rosanna Tofalo¹, Aldo Corsetti²

Affiliations

¹ Faculty of BioScience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, 64100, Teramo (TE), Italy. rtofalo@unite.it.
² Faculty of BioScience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, 64100, Teramo (TE), Italy.

PMID: 28710766
DOI: 10.1007/978-1-4939-7187-9_18

Abstract

RAPD PCR is a sensitive and reliable approach useful for the detection of DNA lesions due to environmental contaminants. In addition, this method is cost-effective, and can be performed in any laboratory having a DNA thermocycler and gel electrophoresis system. Here, we describe its application to identify genotoxin-induced DNA damage in foodborne bacteria. DNA alterations are detected through the analysis of electrophoresis profiles with the appearance or disappearance of new bands as compared to the non-mutated control. The described RAPD PCR procedure takes 6 h for completion. It uses small amounts of DNA and can reveal even low mutation rates.

Keywords: Bacterial foodborne model; DNA damage; Genotoxin; Mutation; RAPD-PCR.

Publication types

Evaluation Study

MeSH terms

DNA Damage*
DNA, Bacterial / genetics*
Enterococcus faecium / drug effects
Enterococcus faecium / genetics*
Lactobacillus plantarum / drug effects
Lactobacillus plantarum / genetics*
Mutagens / toxicity
Mutation
Mutation Rate
Random Amplified Polymorphic DNA Technique / methods*
Risk Assessment

Substances

DNA, Bacterial
Mutagens