Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

Nat Commun. 2017 Jul 13:8:15993. doi: 10.1038/ncomms15993.

Abstract

Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • CRISPR-Cas Systems*
  • Chromatin Assembly and Disassembly*
  • DEAD-box RNA Helicases / metabolism
  • HEK293 Cells
  • Humans
  • K562 Cells
  • Promoter Regions, Genetic
  • beta-Globins / genetics

Substances

  • beta-Globins
  • DDX17 protein, human
  • Ddx5 protein, human
  • DEAD-box RNA Helicases