Cancer Res. 1985 Oct;45(10):5064-9.

Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype.

Lathan B, Edwards DP, Dressler LG, Von Hoff DD, McGuire WL.

Abstract

A monoclonal antibody (IgG1) has been prepared that specifically detects Chinese hamster ovary cells expressing a multidrug-resistant phenotype. This antibody recognizes the membrane P-glycoprotein (Mr 170,000) associated with drug resistance as determined by enzyme-linked immunosorbent assay with purified P-glycoprotein and by Western blot analysis of cell extracts from drug-resistant and drug-sensitive cells. By immunofluorescence methods, the antibody also reacts strongly with viable and ether:ethanol-fixed resistant cells but does not react with the parent drug-sensitive cell line. Thus, this antibody can bind with live cells allowing discrimination by immunohistochemistry between drug-resistant and drug-sensitive Chinese hamster ovary cells.

PMID:: 2862990; [PubMed - indexed for MEDLINE]

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Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodies. [Nature. 1985]
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Kartner N, Evernden-Porelle D, Bradley G, Ling V. Nature. 1985 Aug 29-Sep 4; 316(6031):820-3.
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Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype.

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