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Gene. 1988 Dec 15;73(1):245-50.

Jekyll, a family of phage-plasmid shuttle vectors.

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  • 1European Molecular Biology Laboratory, Heidelberg, F.R.G.

Abstract

A series of shuttle vectors has been constructed, which consist of a plasmid carrying a polylinker sequence and an M13 origin integrated into a lambda vector. A short direct repeat flanking the plasmid allows plasmid excision by homologous recombination. Sequences are cloned into unique restriction sites within the plasmid, and can be recovered either in phage or plasmid form, or can be packaged further as single-stranded DNA phage. These vectors therefore combine the efficiency of phage lambda cloning and screening with the ease of handling or analysing plasmid or M13 clones.

PMID:
2854093
[PubMed - indexed for MEDLINE]
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