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    J Biochem. 1988 Jun;103(6):954-61.

    Construction of a human cytochrome c gene and its functional expression in Saccharomyces cerevisiae.

    Tanaka Y, Ashikari T, Shibano Y, Amachi T, Yoshizumi H, Matsubara H.

    Institute for Fundamental Research, Suntory, Ltd., Osaka.

    The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized.

    PMID: 2844747 [PubMed - indexed for MEDLINE]

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