Identification of enzymes involved in the mevalonate pathway of Flavobacterium johnsoniae

Biochem Biophys Res Commun. 2017 Jun 3;487(3):702-708. doi: 10.1016/j.bbrc.2017.04.120. Epub 2017 Apr 23.

Abstract

The mevalonate pathway is prevalent in eukaryotes, archaea, and a limited number of bacteria. This pathway yields the fundamental precursors for isoprenoid biosynthesis, i.e., isopentenyl diphosphate and dimethylally diphosphate. In the downstream part of the general eukaryote-type mevalonate pathway, mevalonate is converted into isopentenyl diphosphate by the sequential actions of mevalonate kinase, phosphomevalonate kinase, and diphosphomevalonte decarboxylase, while a partial lack of the putative genes of these enzymes is sometimes observed in archaeal and bacterial genomes. The absence of these genes has led to the recent discovery of modified mevalonate pathways. Therefore, we decided to investigate the mevalonate pathway of Flavobacterium johnsoniae, a bacterium of the phylum Bacteroidetes, which is reported to lack the genes of mevalonate kinase and phosphomevalonate kinase. This study provides proof of the existence of the general mevalonate pathway in F. johnsoniae, although the pathway involves the kinases that are distantly related to the known enzymes.

Keywords: Diphosphomevalonate decarboxylase; Isoprenoid; Mevalonate kinase; Mevalonate pathway; Phosphomevalonate kinase.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Chromosome Mapping / methods
  • Conserved Sequence / genetics
  • Evolution, Molecular*
  • Flavobacterium / enzymology*
  • Flavobacterium / genetics*
  • Genome, Bacterial / genetics
  • Mevalonic Acid / metabolism*
  • Phosphotransferases / genetics*
  • Phosphotransferases / metabolism
  • Signal Transduction / genetics*
  • Species Specificity

Substances

  • Bacterial Proteins
  • Phosphotransferases
  • Mevalonic Acid