Chemical constituents from Gueldenstaedtia verna and their anti-inflammatory activity

Nat Prod Res. 2018 May;32(10):1145-1149. doi: 10.1080/14786419.2017.1320795. Epub 2017 Apr 25.

Abstract

A new triterpenoid glycoside (1) and 15 known compounds (2-16) were isolated from the whole plants of Gueldenstaedtia verna. The new compound (1) was identified as complogenin 22-O-β-d-glucopyranoside by extensive spectroscopic techniques including 1D (1H and 13C) and 2D NMR experiments (HSQC, HMBC and NOESY), HR-DART-MS and chemical methods. Most of the isolates were evaluated for their inhibitory activities on LPS-induced NO production in RAW 264.7 cells. The inhibitory effects of the active compounds, sulphuretin (8) and (22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol (13), on the production of pro-inflammatory mediators (including IL-6, IL1β and PGE2) were further estimated in vitro by ELISA in RAW 264.7 macrophages.

Keywords: Gueldenstaedtia verna; anti-inflammatory; inhibition of NO production; triterpenoid glycoside.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / chemistry
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Dinoprostone / metabolism
  • Fabaceae / chemistry*
  • Glycosides / chemistry
  • Glycosides / pharmacology*
  • Inflammation Mediators / metabolism
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Magnetic Resonance Spectroscopy
  • Mice
  • Molecular Structure
  • Nitric Oxide / metabolism
  • RAW 264.7 Cells
  • Triterpenes / chemistry
  • Triterpenes / pharmacology*

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Glycosides
  • IL1B protein, mouse
  • Inflammation Mediators
  • Interleukin-1beta
  • Interleukin-6
  • Triterpenes
  • Nitric Oxide
  • Dinoprostone