Draft Genome Sequence of a Biosurfactant-Producing Bacillus subtilis UMX-103 Isolated from Hydrocarbon-Contaminated Soil in Terengganu, Malaysia

Curr Microbiol. 2017 Jul;74(7):803-805. doi: 10.1007/s00284-017-1249-3. Epub 2017 Apr 17.

Abstract

The draft genome here presents the sequence of Bacillus subtilis UMX-103. The bacterial strain was isolated from hydrocarbon-contaminated soil from Terengganu, Malaysia. The whole genome of the bacterium was sequenced using Illumina HiSeq 2000 sequencing platform. The genome was assembled using de novo approach. The genome size of UMX-103 is 4,234,627 bp with 4399 genes comprising 4301 protein-coding genes and 98 RNA genes. The analysis of assembled genes revealed the presence of 25 genes involved in biosurfactant production, where 14 of the genes are related to biosynthesis and 11 of the genes are in the regulation of biosurfactant productions. This draft genome will provide insights into the genetic bases of its biosurfactant-producing capabilities.

MeSH terms

  • Bacillus subtilis / classification
  • Bacillus subtilis / genetics
  • Bacillus subtilis / isolation & purification*
  • Bacillus subtilis / metabolism*
  • Base Sequence
  • Genome, Bacterial*
  • Hydrocarbons / analysis
  • Hydrocarbons / metabolism*
  • Malaysia
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Soil Microbiology
  • Soil Pollutants / analysis
  • Soil Pollutants / metabolism*
  • Surface-Active Agents / metabolism*

Substances

  • Hydrocarbons
  • Soil Pollutants
  • Surface-Active Agents