Abstract
We report the cloning of murE and murF genes and the identification of their gene products. The murE and murF genes encode diaminopimelic acid- and D-alanyl-D-alanine-adding enzymes, respectively, and both genes are involved in cell wall peptidoglycan biosynthesis in Escherichia coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics
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Bacterial Proteins / genetics*
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Cell Wall / enzymology
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Cell Wall / metabolism
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Chloramphenicol O-Acetyltransferase
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DNA Restriction Enzymes
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Diaminopimelic Acid / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli / ultrastructure
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Genes, Bacterial
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Genetic Complementation Test
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Multigene Family
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Peptidoglycan / biosynthesis*
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Protein Precursors / metabolism
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Transcription, Genetic
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Uridine Diphosphate N-Acetylmuramic Acid / analogs & derivatives
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Uridine Diphosphate N-Acetylmuramic Acid / metabolism
Substances
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Bacterial Proteins
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Peptidoglycan
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Protein Precursors
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Uridine Diphosphate N-Acetylmuramic Acid
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UDP-N-acetylmuramic acid pentapeptide
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Diaminopimelic Acid
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Acetyltransferases
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Chloramphenicol O-Acetyltransferase
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DNA Restriction Enzymes