Phylogenetic analysis of family Neisseriaceae based on genome sequences and description of Populibacter corticis gen. nov., sp. nov., a member of the family Neisseriaceae, isolated from symptomatic bark of Populus × euramericana canker

Yong Li; Han Xue; Sheng-Qi Sang; Cai-Li Lin; Xi-Zhuo Wang

doi:10.1371/journal.pone.0174506

Phylogenetic analysis of family Neisseriaceae based on genome sequences and description of Populibacter corticis gen. nov., sp. nov., a member of the family Neisseriaceae, isolated from symptomatic bark of Populus × euramericana canker

PLoS One. 2017 Apr 13;12(4):e0174506. doi: 10.1371/journal.pone.0174506. eCollection 2017.

Authors

Yong Li¹, Han Xue¹, Sheng-Qi Sang², Cai-Li Lin¹, Xi-Zhuo Wang¹

Affiliations

¹ The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing, China.
² Agricultural High and New Technology Development Center of Puyang, Puyang, China.

Abstract

Two Gram-stain negative aerobic bacterial strains were isolated from the bark tissue of Populus × euramericana. The novel isolates were investigated using a polyphasic approach including 16S rRNA gene sequencing, genome sequencing, average nucleotide identity (ANI) and both phenotypic and chemotaxonomic assays. The genome core gene sequence and 16S rRNA gene phylogenies suggest that the novel isolates are different from the genera Snodgrassella and Stenoxybacter. Additionally, the ANI, G+C content, main fatty acids and phospholipid profile data supported the distinctiveness of the novel strain from genus Snodgrassella. Therefore, based on the data presented, the strains constitute a novel species of a novel genus within the family Neisseriaceae, for which the name Populibacter corticis gen. nov., sp. nov. is proposed. The type strain is 15-3-5T (= CFCC 13594T = KCTC 42251T).

MeSH terms

Genome, Bacterial / physiology*
Neisseriaceae / genetics*
Neisseriaceae / isolation & purification
Phylogeny*
Plant Bark / microbiology*
Populus / microbiology*

Grants and funding

This study was funded by the National Natural Science Foundation of China (31570640) and National Infrastructure of Microbial Resources (NIMR-2017-7). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.