HIV-1 susceptibility of transgenic rat-derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes

Genes Cells. 2017 May;22(5):424-435. doi: 10.1111/gtc.12486. Epub 2017 Mar 22.

Abstract

We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV-1 and supported production of infectious progeny virus. By contrast, both rat primary CD4+ T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell-derived virus was lower than that of human T cell-derived virus.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / virology
  • Cell Line
  • Cells, Cultured
  • Cyclin T / genetics
  • Cyclin T / metabolism*
  • Disease Susceptibility
  • Exportin 1 Protein
  • HIV Infections / immunology*
  • HIV-1 / pathogenicity
  • Humans
  • Karyopherins / genetics
  • Karyopherins / metabolism*
  • Macrophages / immunology*
  • Macrophages / virology
  • Rats
  • Receptors, CXCR4 / genetics
  • Receptors, CXCR4 / metabolism
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism*

Substances

  • Cyclin T
  • Karyopherins
  • Receptors, CXCR4
  • Receptors, Cytoplasmic and Nuclear