Draft genome sequence of multidrug-resistant Salmonella enterica serovar Brancaster strain PS01 isolated from chicken meat, Malaysia

Pui San Chin; Choo Yee Yu; Geik Yong Ang; Wai-Fong Yin; Kok-Gan Chan

doi:10.1016/j.jgar.2016.12.017

Draft genome sequence of multidrug-resistant Salmonella enterica serovar Brancaster strain PS01 isolated from chicken meat, Malaysia

J Glob Antimicrob Resist. 2017 Jun:9:41-42. doi: 10.1016/j.jgar.2016.12.017. Epub 2017 Mar 12.

Authors

Pui San Chin¹, Choo Yee Yu², Geik Yong Ang², Wai-Fong Yin¹, Kok-Gan Chan³

Affiliations

¹ Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
² Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
³ Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: kokgan@um.edu.my.

PMID: 28300643
DOI: 10.1016/j.jgar.2016.12.017

Abstract

Objectives: Salmonella spp. represent one of the main diarrhoeal pathogens that are transmitted via the food supply chain. Here we report the draft genome sequence of a multidrug-resistant Salmonella enterica serovar Brancaster (PS01) that was isolated from poultry meat in Malaysia.

Methods: Genomic DNA was extracted from Salmonella strain PS01 and was sequenced using an Illumina HiSeq 2000 platform. The generated reads were de novo assembled using CLC Genomics Workbench. The draft genome was annotated and the presence of antimicrobial resistance genes was identified.

Results: The 5 036 442bp genome contains various antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, fosfomycin, macrolides, phenicols, sulphonamides, tetracyclines and trimethoprim. The β-lactamase gene bla_TEM-176 encoding TEM-176 was also found in this strain.

Conclusions: The genome sequence will aid in the understanding of drug resistance mechanisms in foodborne Salmonella Brancaster and highlights the need to ensure the judicious use of antibiotics in animal husbandry as well as the importance of implementing proper food handling and preparation practices.

Keywords: Brancaster; Chicken; Food; Resistance; Salmonella enterica; Whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens
Computational Biology
Drug Resistance, Bacterial
Genes, Bacterial
Genome*
High-Throughput Nucleotide Sequencing
Malaysia
Meat / microbiology*
Molecular Sequence Annotation
Salmonella enterica / classification
Salmonella enterica / genetics*
Sequence Analysis, DNA*
Serogroup