J Bacteriol. 1988 Jan;170(1):352-8.

Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion.

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Department of Microbiology and Immunology, University of California, San Francisco 94143.

Abstract

Transposon Tn7 transposes at high frequency to a specific site, attTn7, in the Escherichia coli chromosome. We devised a quantitative assay for Tn7 transposition in which Tn7-end derivatives containing the cis-acting transposition sequences of Tn7 transpose from a bacteriophage lambda vector upon infection into cells containing the Tn7-encoded transposition proteins. We used this assay to identify a 68-base-pair DNA segment containing the sequences essential for attTn7 target activity. This segment is positioned asymmetrically with respect to the specific point of Tn7 insertion in attTn7 and lacks obvious homology to the sequences at the ends of Tn7 which participate directly in transposition. We also show that some sequences essential for attTn7 target activity are contained within the protein-coding sequence of a bacterial gene.

PMID:: 2826397; [PubMed - indexed for MEDLINE]
PMCID:: PMC210649

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Research Support, U.S. Gov't, P.H.S.

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GENBANK/M18980

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Addgene: plasmid: pRM2 insert: attTn7 - Neuroscience Information Framework

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Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion. [J Bacteriol. 1988]
Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion.
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Cited by 27 PubMed Central articles

Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7. [Mob DNA. 2010]
Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7.
Mitra R, McKenzie GJ, Yi L, Lee CA, Craig NL. Mob DNA. 2010 Jul 23; 1(1):18. Epub 2010 Jul 23.
Novel insights about class 2 integrons from experimental and genomic epidemiology. [Antimicrob Agents Chemother. 2...]
Novel insights about class 2 integrons from experimental and genomic epidemiology.
Ramírez MS, Piñeiro S, Centrón D, Argentinian Integron Study Group. Antimicrob Agents Chemother. 2010 Feb; 54(2):699-706. Epub 2009 Nov 16.
Transposition into replicating DNA occurs through interaction with the processivity factor. [Cell. 2009]
Transposition into replicating DNA occurs through interaction with the processivity factor.
Parks AR, Li Z, Shi Q, Owens RM, Jin MM, Peters JE. Cell. 2009 Aug 21; 138(4):685-95.

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