Abstract
The mcr-1 gene was detected in 5.11% (58/1136) of Escherichia coli isolates of chicken origin from 13 provinces in China. A novel mcr-1 variant, named mcr-1.3, encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain. An mcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), was identified. The transfer of pHeN867 led to a 32-fold increase in the MIC of colistin in the recipient, exhibiting an effect on colistin resistance that was similar to that of mcr-1.
Keywords:
E. coli; colistin resistance; mcr-1.3; plasmid.
Copyright © 2017 American Society for Microbiology.
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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Chickens / microbiology*
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China
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Colistin / pharmacology*
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Drug Resistance, Bacterial / genetics
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Escherichia coli / drug effects
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli Infections / drug therapy
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Escherichia coli Infections / veterinary
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Escherichia coli Proteins / genetics*
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Gene Transfer, Horizontal
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Microbial Sensitivity Tests
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Plasmids / genetics
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Poultry Diseases / microbiology*
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Sequence Analysis, DNA
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Escherichia coli Proteins
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MCR-1 protein, E coli
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beta-Lactamases
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beta-lactamase CTX-M, E coli
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Colistin