Colistin Resistance Gene mcr-1 and Its Variant in Escherichia coli Isolates from Chickens in China

Antimicrob Agents Chemother. 2017 Apr 24;61(5):e01204-16. doi: 10.1128/AAC.01204-16. Print 2017 May.

Abstract

The mcr-1 gene was detected in 5.11% (58/1136) of Escherichia coli isolates of chicken origin from 13 provinces in China. A novel mcr-1 variant, named mcr-1.3, encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain. An mcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), was identified. The transfer of pHeN867 led to a 32-fold increase in the MIC of colistin in the recipient, exhibiting an effect on colistin resistance that was similar to that of mcr-1.

Keywords: E. coli; colistin resistance; mcr-1.3; plasmid.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Chickens / microbiology*
  • China
  • Colistin / pharmacology*
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / drug therapy
  • Escherichia coli Infections / veterinary
  • Escherichia coli Proteins / genetics*
  • Gene Transfer, Horizontal
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Poultry Diseases / microbiology*
  • Sequence Analysis, DNA
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • Escherichia coli Proteins
  • MCR-1 protein, E coli
  • beta-Lactamases
  • beta-lactamase CTX-M, E coli
  • Colistin