Effect of thermal pre-treatment on co-digestion of duckweed (Lemna gibba) and waste activated sludge on biogas production

The duckweeds (DW) are considered as a major problem in tropical aquatic system as they grow very fast and produce enormous rich-biomass, which can be harvested for renewable energy operations. But complex lignocellulosic compounds limit their utility in process like anaerobic digestion. This batch study aimed to analyse characteristics (proximate, ultimate and physico-chemical) and possible utility of DW for anaerobic co-digestion with waste activated sludge (WAS) under mesophilic conditions for 35 d. Two sets of experiment were tested: substrate with and without thermal pre-treatment. Five combinations of DW: WAS (70:20, 60:20, 50:20, 40:20 and 30:20%) were established and biomethanation along with changes in pH, volatile solids (VS), volatile fatty acids (VFAs), and soluble chemical oxygen demand (sCOD) of digestate were recorded. The total CH₄ yield (mL CH₄ g^-1 VS) ranged between 60 and 468 for pre-treated, and 9 and 76 for non-pre-treated. The maximum CH₄ yield was 468 mL CH₄g^-1 VS in DW: WAS (50:20). Thermally treated setups, showed about 13-, 24.1-, 21.1-, 1.4-, and 2.3-fold higher CH₄ than non-treated setups. The treated mixtures showed high reduction of _SCOD (>41-96) and VS (>59-98%) in co-digesters. The high degree of Gompertz curve fitting (R² > 0.99) has suggested pre-treatment of substrate for optimal outputs of co-digester. Based on results obtained, it is suggested that DW (50-60% in digester) can be used as renewable energy resource for biomethanation process after thermal pre-treatment.