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1: Proc Natl Acad Sci U S A. 1987 Oct;84(19):6677-81.Click here to read Links

Molecular cloning and amino acid sequence of leukotriene A4 hydrolase.

Funk CD, Rådmark O, Fu JY, Matsumoto T, Jörnvall H, Shimizu T, Samuelsson B.

Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.

A cDNA clone corresponding to leukotriene A4 hydrolase was isolated from a human lung lambda gt11 expression library by immunoscreening with a polyclonal antiserum. Several additional clones from human lung and placenta cDNA lambda g11 libraries were obtained by plaque hybridization with the 32P-labeled lung cDNA clone. One of these clones has an insert of 1910 base pairs that contains the complete protein-coding region. From the deduced primary structure, leukotriene A4 hydrolase is a 610 amino and protein with a calculated molecular weight of 69,140. No apparent homologies with microsomal epoxide hydrolases were found. RNA blot analysis indicated substantial amounts of a discrete mRNA of approximately equal to 2250 nucleotides in lung tissue and leukocytes.

PMID: 2821541 [PubMed - indexed for MEDLINE]

PMCID: PMC299146