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Yao Xue Xue Bao. 1989;24(4):275-9.

[Determination of berberine and palmatine in cortex phellodendron and Chinese patent medicines by HPLC].

[Article in Chinese]

Abstract

Berberine (1) and palmatine (2) in cortex phellodendron and Chinese patent medicines were determined by HPLC. The optimal composition of mobile phase CH3COOEt-HCOOH-EtOH (15:3:2) for HPLC separation of berberine and palmatine was successfully determined by using window diagram technique. Detection wavelength was 346nm. Flow rate: 1.5 ml/min. Calibration graphs for 1 and 2 were rectilinear for 0.06-0.32 micrograms and 0.12-0.32 micrograms respectively. The average recoveries of the two corresponding components were found to be over 96% and their coefficients of variation were below 5%.

PMID:
2816389
[PubMed - indexed for MEDLINE]
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