Virome of US bovine calf serum

Mohammadreza Sadeghi; Beatrix Kapusinszky; Danielle M Yugo; Tung Gia Phan; Xutao Deng; Isis Kanevsky; Tanja Opriessnig; Amelia R Woolums; David J Hurley; Xiang-Jin Meng; Eric Delwart

doi:10.1016/j.biologicals.2016.12.009

Virome of US bovine calf serum

Biologicals. 2017 Mar:46:64-67. doi: 10.1016/j.biologicals.2016.12.009. Epub 2017 Jan 15.

Authors

Affiliations

¹ Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Virology, University of Helsinki, Finland.
² Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.
³ Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
⁴ Department of Dairy Science, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
⁵ The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, Scotland, UK.
⁶ Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, MS, USA.
⁷ Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
⁸ Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA. Electronic address: delwarte@medicine.ucsf.edu.

Abstract

Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

Keywords: Bosavirus; Cell culture; Metagenomics; Parvovirus.

MeSH terms

Animals
Bocavirus / classification
Bocavirus / genetics
Capsid Proteins / classification
Capsid Proteins / genetics
Cattle / blood*
Cattle / virology*
Genome, Viral / genetics*
Geography
Metagenomics / methods*
Parvoviridae Infections / veterinary
Parvoviridae Infections / virology
Phylogeny
Sequence Analysis, DNA
Serum / virology
Species Specificity
United States
Viral Nonstructural Proteins / classification
Viral Nonstructural Proteins / genetics

Substances

Capsid Proteins
Viral Nonstructural Proteins

Grants and funding

R01 HL105770/HL/NHLBI NIH HHS/United States