Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State

Cell Rep. 2017 Jan 10;18(2):443-453. doi: 10.1016/j.celrep.2016.12.032.

Abstract

One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.

Keywords: Dynamin-2; HIV-1 fusion; advanced light imaging; cell-cell fusion; number and brightness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / immunology
  • Cell Count
  • Cell Fusion
  • Dynamin II / chemistry*
  • Dynamin II / metabolism*
  • Fluorescence Resonance Energy Transfer
  • Genes, Reporter
  • HEK293 Cells
  • HIV-1 / physiology*
  • Humans
  • Hydrazones / metabolism
  • Kinetics
  • Membrane Fusion*
  • Models, Biological
  • Protein Multimerization*
  • Virion / metabolism
  • Virus Internalization

Substances

  • Hydrazones
  • N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide
  • Dynamin II