Chemical Compositions of the Peel Essential Oil of Citrus aurantium and Its Natural Larvicidal Activity against the Malaria Vector Anopheles stephensi (Diptera: Culicidae) in Comparison with Citrus paradisi

Alireza Sanei-Dehkordi; Mohammad Mehdi Sedaghat; Hassan Vatandoost; Mohammad Reza Abai

Chemical Compositions of the Peel Essential Oil of Citrus aurantium and Its Natural Larvicidal Activity against the Malaria Vector Anopheles stephensi (Diptera: Culicidae) in Comparison with Citrus paradisi

J Arthropod Borne Dis. 2016 Oct 4;10(4):577-585. eCollection 2016 Dec.

Authors

Alireza Sanei-Dehkordi¹, Mohammad Mehdi Sedaghat², Hassan Vatandoost², Mohammad Reza Abai²

Affiliations

¹ Department of Medical Entomology and Vector Control, Faculty of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; Social Determinants in Health Promotion Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
² Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 28032110
PMCID: PMC5186748

Abstract

Background: Recently, essential oils and extracts derived from plants have received much interest as potential bio-active agents against mosquito vectors.

Methods: The essential oils extract from fresh peel of ripe fruit of Citrus aurantium and Citrus paradisi were tested against mosquito vector Anopheles stephensi (Diptera: Culicidae) under laboratory condition. Then chemical composition of the essential oil of C. aurantium was analyzed using gas chromatography-mass spectrometry (GC-MS).

Results: The essential oils obtained from C. aurantium, and C. paradisi showed good larviciding effect against An. stephensi with LC₅₀ values 31.20 ppm and 35.71 ppm respectively. Clear dose response relationships were established with the highest dose of 80 ppm plant extract evoking almost 100% mortality. Twenty-one (98.62%) constituents in the leaf oil were identified. The main constituent of the leaf oil was Dl-limonene (94.81).

Conclusion: The results obtained from this study suggest that the limonene of peel essential oil of C. aurantium is promising as larvicide against An. stephensi larvae and could be useful in the search for new natural larvicidal compounds.

Keywords: Anopheles stephensi; Citrus aurantium; Citrus paradisi; Essential oil; Larvicidal activity.