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Mol Biochem Parasitol. 1989 Oct;36(3):217-22.

Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.

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  • 1Department of Pharmacology, Stanford University, CA 94305.

Abstract

A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-specific, repeated DNA fragments. Using W1 as a probe, we have developed a rapid and accurate dot-blot assay for determining the sex of S. mansoni cercariae.

PMID:
2797060
[PubMed - indexed for MEDLINE]
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