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    EMBO J. 1989 Jul;8(7):1981-6.

    The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties.

    Source

    Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.

    Abstract

    We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400.

    PMID:
    2792078
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC401066
    Free PMC Article

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