Transcription factors regulating uspA genes in Catharanthus roseus

Ahmed Bahieldin; Ahmed Atef; Ahmed M Shokry; Saleh Al-Karim; Sanaa G Al Attas; Nour O Gadallah; Sherif Edris; Magdy A Al-Kordy; Sabah M Hassan; Salah Abo-Aba; Fotouh M El-Domyati

doi:10.1016/j.crvi.2016.10.004

Transcription factors regulating uspA genes in Catharanthus roseus

C R Biol. 2017 Jan;340(1):1-6. doi: 10.1016/j.crvi.2016.10.004. Epub 2016 Nov 24.

Authors

Affiliations

¹ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: bahieldin55@gmail.com.
² Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: ahmed_atefaig2@yahoo.com.
³ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt. Electronic address: ahmedshokry1@gmail.com.
⁴ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: Skarim@kau.edu.sa.
⁵ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: sangtas@mit.edu.
⁶ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt. Electronic address: nouromar71@yahoo.com.
⁷ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt; Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), Faculty of Medicine, King Abdulaziz University (KAU), Jeddah, Saudi Arabia. Electronic address: sedris@aucegypt.edu.
⁸ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt.
⁹ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: m_alkordy@yahoo.com.
¹⁰ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Centre, Giza, Egypt. Electronic address: sabmahmoud@yahoo.com.
¹¹ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: fm_domyati@hotmail.com.

PMID: 27890577
DOI: 10.1016/j.crvi.2016.10.004

Abstract

RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethylene-responsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses.

Keywords: BHLH; Co-expression; Erf; RNA-Seq; SqRT-PCR; Transcription factor; WRKY.

MeSH terms

Catharanthus / genetics*
Computer Simulation
Databases, Genetic
Gene Expression Regulation, Plant
Genes, Plant / genetics*
RNA, Plant / genetics
Real-Time Polymerase Chain Reaction
Stress, Physiological / genetics
Transcription Factors / genetics*
Up-Regulation / genetics

Substances

RNA, Plant
Transcription Factors