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    J Immunol. 1989 Sep 15;143(6):1822-5.

    Two better cell lines for making hybridomas expressing specific T cell receptors.

    White J, Blackman M, Bill J, Kappler J, Marrack P, Gold DP, Born W.

    Howard Hughes Medical Institute, Department of Pediatrics, Denver, CO.

    Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.

    PMID: 2778316 [PubMed - indexed for MEDLINE]

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