Anti-allergic effects of His-Ala-Gln tripeptide in vitro and in vivo

Mamoru Tanaka; Hiroyuki Watanabe; Yoshinobu Yoshimoto; Hana Kozai; Takeaki Okamoto

doi:10.1080/09168451.2016.1243984

Anti-allergic effects of His-Ala-Gln tripeptide in vitro and in vivo

Biosci Biotechnol Biochem. 2017 Feb;81(2):380-383. doi: 10.1080/09168451.2016.1243984. Epub 2016 Oct 12.

Authors

Mamoru Tanaka¹, Hiroyuki Watanabe¹, Yoshinobu Yoshimoto², Hana Kozai³, Takeaki Okamoto⁴

Affiliations

¹ a Department of Nutrition , University of Kochi , Kochi , Japan.
² b Department of Physical Therapy , Seirei Christopher University , Hamamatsu , Japan.
³ c College of Bioscience and Biotechnology , Chubu University , Kasugai , Japan.
⁴ d Faculty of Education , Ehime University , Matsuyama , Japan.

PMID: 27728999
DOI: 10.1080/09168451.2016.1243984

Abstract

We examined the inhibitory effects of HAQ (His-Ala-Gln) peptide on type-1 allergy in vitro and in vivo. HAQ peptide inhibited β-hexosaminidase release and intracellular Ca²⁺ levels of rat basophilic leukemia RBL-2H3 cells. Oral administration of a HAQ peptide-added diet (1 mg/mouse/administration) to C3H/HeJ mice for 14 days led to significant suppression of allergic symptoms, but did not reduce allergen-specific IgE or IgG₁.

Keywords: RBL-2H3 cell; anti-allergic effect; mouse model; peptide.

MeSH terms

Administration, Oral
Animals
Anti-Allergic Agents / administration & dosage
Anti-Allergic Agents / pharmacology*
Anti-Allergic Agents / therapeutic use
Calcium / metabolism
Cell Line, Tumor
Hypersensitivity / blood
Hypersensitivity / drug therapy
Immunoglobulin E / blood
Immunoglobulin G / blood
Intracellular Space / drug effects
Intracellular Space / metabolism
Mice
Oligopeptides / administration & dosage
Oligopeptides / pharmacology*
Oligopeptides / therapeutic use
Rats
beta-N-Acetylhexosaminidases / metabolism

Substances

Anti-Allergic Agents
Immunoglobulin G
Oligopeptides
histidyl-alanyl-glutamine
Immunoglobulin E
beta-N-Acetylhexosaminidases
Calcium